Scientist - Recombinant Protein Production & Cell-Based Assays

Scientist - Recombinant Protein Production & Cell-Based Assays

Selvita

Wrocław, Poland

We are seeking a highly motivated Scientist to join our Antibody Discovery team within a CRO environment. The successful candidate will be responsible for the development, optimization and execution of recombinant antibodies and other proteins production workflows across multiple expression systems, as well as the implementation of antibody cell-based assays supporting client research programs.

Key Responsibilities

• Design and optimize expression of recombinant proteins, antibodies and VHH in bacterial and mammalian systems;
• Perform transient transfections and support development of stable expression systems;
• Execute small- to mid-scale protein production;
• Purify proteins using affinity, ion exchange and size exclusion chromatography (ÄKTA/FPLC experience is a plus);
• Perform protein characterization using SDS-PAGE, Western blot and SEC-HPLC;
• Maintain adherent and suspension mammalian cell lines;
• Develop and perform cell-based functional assays (e.g. proliferation, apoptosis);
• Experience with PBMCs or primary blood cells is an advantage;
• Analyze biological data and troubleshoot basic assay variability;
• Plan and execute experiments in line with project goals;
• Maintain accurate laboratory documentation and records;
• Prepare technical reports and participate in project meetings.

Your Background

• MSc or PhD in Biotechnology, Biology, Molecular Biology, Biochemistry, Cell Biology, or a related field;
• Minimum 3 years of hands-on experience in recombinant protein production in academic or industry settings;
• Experience with recombinant protein or antibody production across multiple expression systems;
• Practical knowledge of chromatographic purification techniques and mammalian cell culture;
• Experience in developing and performing cell-based assays;
• Familiarity with protein analytical methods, including ELISA-based assays;
• Strong data analysis and interpretation skills;
• Good command of English, both written and spoken;
• Experience working in a client-facing CRO environment is an advantage;
• Ability to work effectively in cross-functional project teams.