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The project will investigate how tissue stiffness, extracellular matrix composition, mechanosensitive signaling proteins regulate neuron–glioma interaction and tumor proliferation. The broader goal is to identify therapeutic vulnerabilities that arise
Carry out molecular cloning and construct design, including plasmid design, Gibson/Golden Gate assembly, and site-directed mutagenesis; Express proteins using bacterial-, mammalian-cell-based (e.g., E. coli, HEK293), and cell-free protein expression
You will investigate the mechanisms of how pathogen effectors bind to their host targets to promote disease. You will implement a pipeline for identification of host proteins targeted by effectors from Magnaporthe oryzae, the causal agent of the
Design and perform genetic and genomics screens to reconstruct maps of functional interactions across the ERN; Design and carry out functional experiments to mechanistically dissect the basis of identified interactions; Identify physiologically relevant