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This programme of research will investigate candidate pathophysiological mechanisms of susceptibility in takotsubo cardiomyopathy. Specifically, the work of the appointee will focus on (1) the activation of innate and adaptive immunity
Generate and characterize human iPSC and mouse ESC-derived 2D cortical neural stem cells, neurons and 3D cortical organoids carrying ADAT2/ADAT3 pathogenic variants; Assess cellular phenotypes (proliferation, cell cycle dynamics
This project aims to further understand the reprogramming of cells exposed to early and chronic hypoxia in multiple experimental models, with a particular focus on CRISPR and single cell sequencing. The project will involve perturbation experiments in
You'll lead the development and optimisation of workflows for nucleic acid extraction, purification, library preparation, and sample processing within a fluidic device, helping to reduce technical risk and guide the platform from proof-of-concept through
Investigate and evaluate new sample preparation protocols for bottom-up proteomics, including protein digestion, peptide clean-up, enrichment of post-translationally modified peptides, and handling sub-nanogram protein quantities; Optimize protocols for