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This programme of research will investigate candidate pathophysiological mechanisms of susceptibility in takotsubo cardiomyopathy. Specifically, the work of the appointee will focus on (1) the activation of innate and adaptive immunity
You'll lead the development and optimisation of workflows for nucleic acid extraction, purification, library preparation, and sample processing within a fluidic device, helping to reduce technical risk and guide the platform from proof-of-concept through
Investigate and evaluate new sample preparation protocols for bottom-up proteomics, including protein digestion, peptide clean-up, enrichment of post-translationally modified peptides, and handling sub-nanogram protein quantities; Optimize protocols for
Carry out molecular cloning and construct design, including plasmid design, Gibson/Golden Gate assembly, and site-directed mutagenesis; Express proteins using bacterial-, mammalian-cell-based (e.g., E. coli, HEK293), and cell-free protein expression
The aim of the project is to elucidate molecular intra- and extracellular mechanisms of signaling modulating myeloid cells' immune responses. The project is supported by Innosuisse and has a translational goal aiming to pave a path to clinical