AMBER Postdoctoral Fellowship: Cross-scale Imaging of the Invasion and Progression of Toxoplasma Gondii in Human Cells

AMBER Postdoctoral Fellowship: Cross-scale Imaging of the Invasion and Progression of Toxoplasma Gondii in Human Cells

EMBL - European Molecular Biology Laboratory

Grenoble, France

EMBL is Europe’s life sciences laboratory – an intergovernmental organisation with more than 110 independent research groups and service teams covering the spectrum of molecular biology. It operates across six sites in Heidelberg (headquarters), Barcelona, Cambridge, Grenoble, Hamburg and Rome. Our mission is to perform basic research in molecular biology; train scientists, students and visitors at all levels; offer vital services to scientists in the public and private sectors within the member states; develop new instruments and methods; and engage actively in technology transfer.

This postdoctoral position is part of the EU cofund research project AMBER, Advanced Multiscale Biological imaging using European Research infrastructures, will address scientific and sectoral gaps in biological imaging ranging from molecular, through cellular, to tissue, organ and organism levels of organisation, and is coordinated by LINXS Institute of advanced Neutron and X-ray Science. AMBER is funded by the EU Marie Skłodowska-Curie (MSCA) COFUND scheme.

Your work may include clinical and biomedical projects. It may also include technique development work aimed at combining imaging techniques and data analysis to provide a more integrated picture of life processes in the context of health and disease. To be a postdoc fellow at the AMBER programme you will get unprecedented medical, biological, and methodological capabilities, with a profound potential impact for Europe’s next generation of research and researchers. When you have completed the AMBER programme you will be extraordinarily well equipped to further your career in academia, at infrastructures, in the health and MedTech sectors, and beyond.

Your role

Toxoplasma belongs to the phylum Apicomplexa, which includes numerous important human and animal pathogens such as Plasmodium species, Cryptosporidium, and Neospora caninum. The protozoan parasite T. gondii is the etiologic agent of toxoplasmosis, a usually mild disease in immunocompetent humans that can turn into a major threat to the unborn and to immunocompromised people, with acquired immunodeficiency syndrome or under chemo- and graft rejection therapies. Toxoplasma is an obligate parasite that develops and proliferates inside a surrogate host cell.

We propose to perform a mesoscale exploration of the invasion and persistence of T. gondii in its host cell. This will be performed for the first time on the same sample using a support that we are developing that is compatible with light, X-ray and electron imaging. We aim to routinely collect X-ray tomographic data on whole infected human cells in order to target regions of interest (e.g interface between parasite and host cell organelles) for FIB milling and cryoET data collection. Phase contrast tomography of these samples is already providing unprecedented insight into the infection process, correlation with high resolution tomography on targeted regions will be a sea change in Toxoplasma research and will potentially allow molecular details of infection to be determined via sub-tomogram averaging of complexes in regions previously identified by phase contrast imaging. This project poses unique challenges in correlating 3D volumes at different scales and resolutions. In collaboration with the Hakimi lab (IAB, Grenoble), beamline ID16A at the ESRF and the EMBL imaging centre in Heidelberg, we are developing the tools required for this project and are starting to generate the first data sets. This is an opportunity to drive innovative developments in data processing in correlative imaging applied to an important infectious agent.

You have

• a maximum 8 years after a doctoral degree (PhD), as required by the project Grant Agreement signed with the European Commission;
• at least one original publication in a peer-reviewed journal;
• a background in the relevant methods;
• a complete application package submitted through the AMBER portal (including CV and detailed research plan), and finally;
• strict compliance with the MSCA mobility rule that the researcher must not have resided or carried out his/her main activity (work, studies, etc.) in the host organisation's.

Contract length: 3 years

What else you need to know

AMBER has six core partners: Lund University/MAX IV, Sweden, the European Spallation Source (ESS), Sweden, the European Molecular Biology Laboratory (EMBL), Institut Laue-Langevin (ILL), France, the International Institute of Molecular Mechanisms and Machines, (IMOL), Poland, and the Leicester Institute of Structural and Chemical Biology, United Kingdom.

Around 20 postdocs will be recruited in the Fourth call 2025, with each fellowship lasting 36 months.

If you are interested in applying for more than one position (maximum 3) you must be prepared to make a full application for each individual position.

Applications shall be written in English and compiled into a PDF-file containing:

A Curriculum Vitae (europass format). This is created by registering and using the Europass portal here: https://europa.eu/europass/en/create-europass-cv. Your CV can then be exported to a PDF file that you use in your application. A detailed research plan including any foreseen secondments (candidates can suggest more than the mandated one, they can also suggest their own secondments), schools and conferences as well as a templated budget plan. (as concise as possible, recommended about 3-5 pages, but at an absolute maximum 10 pages. The research plan should include a half page of summary/abstract.

Additional texts to include in the PDF are:

• Letter of Commitment from any additional secondment partners the candidate wishes to bring onboard;
• Evidence of English proficiency (minimum CEFR B22, also checked at interview);
• A draft Individual Career Development Plan (ICDP);
• Two reference letters;
• Any additional documents to support the application;
• Ethical questionnaire (HE ethics checklist + research ethics commitment) link will be provided at the “For applicants” page, here: https://www.ambercofund.eu/for-applicants.

In addition, the application will require:

Any candidate can apply for maximum of 3 positions. You must apply for each individual position. A list with order of preference of positions should be sent to the AMBER management amber@linxs.lu.se.

The template can be downloaded from the “For applicants” page, here: https://www.ambercofund.eu/for-applicants.

For more information about AMBER, application and evaluation process etc. please visit: https://www.ambercofund.eu.

You can always e-mail and ask the AMBER management questions at: amber@linxs.lu.se.

Professional development support

The EMBL Fellows’ Career Service provides support and guidance to predoctoral and postdoctoral fellows across all six EMBL’s sites.

Working with a dedicated Careers Advisor, this invaluable service will help you to take informed decisions about your career planning both in the short and longer term. Whether your main interest is pursuing a career path in academia, exploring opportunities in industry or exploring an independent venture, the EMBL Fellows’ Career Service with provide you with a portfolio of activities and resources to help you.

To find out more please visit - EMBL-fellows-career-service.

Why join us

Join a culture of innovation:

We are located on the Wellcome Genome Campus, alongside other prominent research and biotech organisations, and surrounded by beautiful Cambridgeshire countryside. This is a highly collaborative and inclusive community where our employees enjoy a relaxed atmosphere. We are committed to ensuring our employees feel valued, supported and empowered to reach their professional potential.

Enjoy lots of employee benefits:

• Financial incentives: Monthly family and child allowances, generous stipend reviewed yearly, pension scheme, death benefit and unemployment insurances;
• Flexible working arrangements including hybrid working patterns;
• Private medical insurance for you and your immediate family;
• Generous time off: 30 days annual leave per year in addition to public holidays;
• Campus life: Free shuttle bus to and from work (Heidelberg), on-site library, subsidised on-site gym and cafeteria, casual dress code, extensive sports and social club activities (on campus and remotely);
• Family benefits: Kindergarten (Heidelberg), 10 days of child sick leave, paid maternity & parental leave, holiday clubs on campus and monthly family and child allowances.

What else you need to know

• International applicants: We recruit internationally and successful candidates are offered visa exemptions. Read more on our page for international applicants;
• Diversity and inclusion: At EMBL, we strongly believe that inclusive and diverse teams benefit from higher levels of innovation and creative thought. We encourage applications from women, LGBTQ+ & individuals from all nationalities;
• Job location: All our fellowships are based on-site (for at least part of each week). If you are living overseas, you will receive a generous relocation package to support you;
• EMBL is a signatory of DORA. Find out how we apply DORA principles to our recruitment and performance assessment processes here;
• How to apply: To apply please submit a cover letter and a CV through our online system. Applications will close at 23:59 CET on the date shown below. We aim to provide a response within two weeks after the closing date.

Closing Date: 08/09/2025

Apply NowDeadline 8 September