Technician/Senior Technician (Magda Bienko Group)

HT - Human Technopole

Technician/Senior Technician (Magda Bienko Group)

HT - Human Technopole

Milan, Italy

Job description


Human Technopole (HT) is a new interdisciplinary life science research institute, created and supported by the Italian Government, with the aim of developing innovative strategies to improve human health. HT is composed of five Centres: Computational Biology, Structural Biology, Genomics, Neurogenomics and Health Data Science. The Centres work together to enable interdisciplinary research and to create an open, collaborative environment that will help promote life science research both nationally and internationally.

The Bienko Group at HT ( focuses on understanding the fundamental principles that regulate how chromatin is spatially organized in the nucleus of mammalian cells and how the three-dimentional (3D) organization of the genome is affected by alterations in its sequence, such as those commonly encountered in human cancers.

The group is now inviting outstanding candidates with an excellent background in molecular biology/biotechnology and proven track record in various laboratory techniques to apply for a position as Technician or Senior Technician depending on the candidate’s level of experience.

The successful candidate will be embedded in a multi-cultural and interdisciplinary team and take part in the lab’s efforts in engineering cells with rewired karyotypes, studying the effects of karyotypic changes on genome architecture and gene expression, and developing new methods to study the 3D genome in single cells.

She/he will have the following responsibilities:

  • Leverage CRISPR-Cas technologies to engineer cell lines carrying balanced chromosomal translocations;
  • Characterize the engineered cell lines using a variety of assays, including methods developed by the group, such as GPSeq (Girelli et al, Nat Biotechnol 2020);
  • Apply GPSeq and other sequencing-based methods established by the group in the frame of scientific collaborations with other research groups nationally and internationally;
  • Support the group in various tasks, including but not limited to: (i) overseeing reagent and small equipment purchases; (ii) setting up and maintaining a lab reagent inventory; (iii) setting up and maintaining working routines and standard operating procedures (SOP); (iv) networking with facilities at HT.

Job requirements

  • M.Sc. or Ph.D. degree in Molecular Biology, Biotechnology, Biomedicine, or equivalent;
  • Extensive theoretical knowledge and documented hands-on experience in mammalian cell culture/transfection/transduction and basic molecular biology/biochemistry/microscopy techniques (DNA/RNA purification, PCR, reverse transcription, in vitro transcription, cloning, western blotting, widefield and/or confocal immunofluorescence microscopy);
  • Proficiency with various omic methods that are particularly relevant to the research focus of the group (e.g., RNA-seq, ChIP-seq, ATAC-seq, Hi-C) and/or with CRISPR-Cas technologies will be considered a strong advantage;
  • Ability to independently organize her/his own work routine and to flexibly adapt her/his weekly schedule to emerging requests;
  • Strong problem-solving and forward-looking personality, i.e., she/he is motivated to solve obstacles that might present on the path to achieving a goal, enjoys troubleshooting, is not afraid of seeking for help, and strongly believes that a solution to a problem can be found even if this means revising the original goal/plan (never compromising scientific rigor and quality);
  • Strong collaborative attitude, i.e., she/he is a strong team player and feels that the success of the whole team will only come from all its members being actively engaged and helping each other;
  • Fluency in oral and written English;
  • Special consideration will be given to candidates who are part of the protected categories list, according to L. 68/99.

Additional information

HT offers a highly collaborative, international culture to foster top quality, interdisciplinary research by promoting a vibrant environment consisting of independent research groups with access to outstanding graduate students, postdoctoral fellows, and core facilities.

HT is an inclusive employer that fosters diversity and engages systematically to ensure that equal employment opportunities are provided without regard to age, race, creed, religion, sex, disability, medical condition, sexual orientation, gender identity or expression, national or ethnic origin or any other legally recognized status entitled to protection under applicable laws. HT offers attractive conditions and benefits appropriate to a leading, internationally competitive, research organization that promotes a collegial and open atmosphere and favors flexible and person-tailored working conditions to balance work with personal life.

The Human Technopole Foundation is based in Milan, recently named the city with the best quality of life in Italy (

Please apply by sending a CV and a 1-page motivation letter in English only through the dedicated area below (Apply now) and the name and contact details of at least 2 potential referees. In the motivation letter, please emphasize your prior lab work and how you think you can contribute to the success of the Bienko Group and Human Technopole.

For specific enquires concerning the role only, please contact Magda Bienko, Group Leader.

Personalized Recruitee email generated by platform to be inserted.

Number of positions offered: 1
Contract offered: CCNL Chimico Farmaceutico, 5 yrs fixed-term - Employee level

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