The main aim of this project is to determine how the spatial organization of the genome changes upon replication stress and how these changes help to protect the cell from the harmful consequences of this stress. By using the most modern methods relying on nextgeneration sequencing, we will compare the three-dimensional structure of the genome in correctly replicating cells and those exposed to replication stress. DNA regions that are particularly susceptible to stress-related damage will also be identified allowing to generate a comprehensive, genome-wide map of fragile sites in human cells. It will enable us to determine the characteristic features of those sites and subsequently to get novel insights into the causes of their breakage. In addition, we will investigate the consequences of permanent 3D changes emerging in cells due to replication stress in terms of their potential contribution to the development of cancer. Based on the obtained results, a computational model, determining genomic sites, where the damage may initiate or stimulate the development of cancer, will be created. Additionally, we will develop new tools enabling modeling of structural elements of DNA such as single DNA loops and dynamics of their formation. The execution of the project will significantly broaden our understanding of the mutual relations between replication stress, the spatial structure of the genome, and genetic instability. In addition, the obtained results can potentially be used in the diagnosis and therapy of diseases associated with chromosome fragility, such as cancer, autism, or mental developmental disorders.
The competition is open to persons who meet the conditions specified in:
We allow for a short (~a few months) modification of the date of starting employment in line with the candidate’s preferences. The possibility of starting employment at a later date shall be agreed upon with the project leader.
The candidate should hold a PhD degree for no longer than 7 years before the date of signing an employment agreement in the project. The PhD degree should be obtained in a country of the EU, EFTA, OECD or nostrified on the date of employment at the latest.