The successful candidate will work in close collaboration with a Postdoc in the lab with an expertise in patch-clamp recordings, genome editing (CRISPR/Cas-9) and 3D-STORM imaging, as well as with the LMB Light-microscopy team. We also closely collaborate with the Cambridge University on studies of the hippocampal synaptic plasticity mechanisms. In this project, we will characterise signalling and nano-positioning of synaptic/dendritic glutamate receptors under baseline conditions and in response to LTP. LTP will be induced by various means such as 2-photon glutamate uncaging onto individual dendritic spines. Towards this wider aim, we have established various state of the art approaches including 3D-STORM, STED and CRISPR/Cas-9 genome editing. A spare electrophysiology rig will be available to the successful candidate. We also aim to compare native AMPARs to reconstituted recombinant receptors.
These should include a PhD in a relevant subject or due to complete PhD within 6 months.
Experience of and ability to perform techniques relevant to the project. Particularly useful would be some of the following: