In Human Immunodeficiency Virus (HIV)-infected individuals on antiretroviral therapy, the persistence of the virus in latently infected cells is thought to be the barrier for cure of infection. The main latent reservoir is constituted of resting memory CD4+ T lymphocytes containing integrated proviruses that are transcriptionally silent. The project is based on our recent progress on the role of novel complex containing LEDGF/p75, Iws1 and Spt6 that participates to post-integration silencing of HIV-1. The candidate will study the role of protein complexes regulating transcription of integrated proviruses.
The project is in collaboration with the lab of Rosemary Kiernan (IGH, Montpellier) to study the molecular mechanisms involved in the establishment and maintenance of HIV latency. Applicants for this position should have strong knowledge and expertise in virology, protein biochemistry, molecular biology and the field of transcription. Main activities will be: cell tissue culture and viral infection in BSL2 and BSL3 environment, recombinant protein and protein complex purification, transcription assays, ChIP assays.