Cryo-EM, High-Resolution SPA and Cryo-Electron Tomography Specialist

Cryo-EM, High-Resolution SPA and Cryo-Electron Tomography Specialist

Institut Pasteur

Paris, France

We are seeking a highly motivated individual to join our Structural Biology team as a specialist to lead and support our cryo-EM, high-resolution SPA and cryo-electron tomography efforts.

Responsibilities

  • Cryo-EM image sample screening and data acquisition;
  • Sample preparation with vitrobot and manual plunger-freezers;
  • Operation and maintenance of cryo-EM-related ancillary equipment;
  • Training, assisting, and overseeing team members in cryo-EM sample preparation;
  • Developing and implementing efficient strategies for cryo-EM sample preparation and data collection;
  • Participating in the development and implementation of new methods/techniques in cryoEM;
  • Managing the unit’s cryo-EM supplies including, but not limited to, organizing the labs, ordering supplies, keeping records, writing reports, and other activities as assigned.

The successful applicant will work in a stimulating academic and cultural environment, and will have the opportunity to contribute as an author to manuscripts. The unit is equipped with cell culture, molecular biology, light and cryo-CLEM technology, as well as will have access to the newly established Nanoimaging Core at Institut Pasteur, headed by Matthijn Vos. The state-of the-art core houses a 300kV Titan Krios equipped with a K3 direct electron detector and energy filter for high resolution structural studies of biological molecules in situ, 2 screening 200 kV Glacios electron microscopes, as well as one Aquilos and all required ancillary equipment for cellular cryo-ET sample preparation.

Required Experience

  • Strong Hands-on experience in negative stain and cryo-EM sample preparation techniques
  • Strong Hands-on experience in operation of vitrobot or equivalent, manual plungers and other related equipment in biological electron microscopy;
  • Hands-on experience in operation of electron cryo TEMs (120, or FEG) microscopes for data acquisition;
  • Hands-on experience in usage of cryo-EM data acquisition software EPU, Serial EM a plus;
  • Bachelor’s degree in a biological science, chemistry, physics or a related field, or an equivalent combination of education, training and experience from which comparable knowledge, skills and abilities have been attained;
  • Requires a minimum of 5+ years of directly related experience to cryo-EM sample preparation.
  • Candidates with a master’s degree, a minimum of 3+ years is required.

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© EuroJobsites 2019

EuroJobsites is a registered company number: 4694396 VAT number: GB 880 9055 04

Registered address: EuroJobsites Ltd, Unit 8, Kingsmill Business Park, Kingston Upon Thames, London, KT1 3GZ, United Kingdom

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