Postdoc (Adjunct) - Proximity-induced SuFEx Ligation for mRNA Biology and Medicine
Postdoc (Adjunct) - Proximity-induced SuFEx Ligation for mRNA Biology and Medicine
University of Warsaw - Centre of New Technologies
Warsaw, Poland
Project description:
The aim of this project is to implement the Sulfur-Fluorine Exchange (SuFEx) reaction into the field of nucleosides, (oligo)nucleotides and mRNA. SuFEx in a novel click-type reaction that takes advantage of exceptional chemical properties of the S(VI)-F bond present in sulfonyl fluorides (R-SO2F), fluorosulfates (RO-SO2F) and sulfamoyl fluorides (RNH-SO2F). Apart from its utility in general organic chemistry SuFEx approach towards biomolecules creates unique opportunity by gaining them new functions and properties through introduction of “sufexable” units into their structure.
Key responsibilities include:
Enzymatic synthesis and purification of modified RNAs, preparation of DNA for IVT experiments, identification of enzymatic reactions products and chemically induced crosslinks, pull-down experiments and preparation of the samples for proteomic analysis, evaluation of the RNA delivery systems using model cell lines.
Profile of candidates/requirements:
The competition is open to persons who meet the conditions specified in:
- Article 113 of the Act of 20 July 2018 Law on higher education and science (Journal of Laws of 2023, item 742 with amendments) and the Statutes of the University of Warsaw;
- Regulations on the allocation of resources for the implementation of tasks financed by the National Centre of Science for OPUS 25 grant;
- The candidate must be a holder of PhD degree in molecular biology, biochemistry, biotechnology or related field;
- Experience in RNA biochemistry will be an advantage.
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